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"Synthetic DNA Reductase"
(Garnett), J. Bioinorg. Chem. V. 59, P. 231 Aug. '95, Lubeck
Garnett McKeen Laboratory,
L.I.H.T. Incubator, University Campus,
25 East Loop Road, Stony Brook,
New York 11790, USA.
Platinum compounds are well established as chemotherapy agents (1). I now report on much safer, water-soluble organo-palladium
complexes, undergoing clinical oncology studies. Structure analyses included FTIR, column separations, osmometry,
and elemental analysis. The core unit of the polymer consists of a palladium bonded to both end groups of a lipoic
acid: two sulfurs of the thiolane ring, and the carboxyl oxygens of the pentanoic chain. Three such core units
become trimerized when complexed with a single thiamine. Column separations show further polymerization of the
trimers is possible, and this appears due to the reducing environment.
These compounds appear to have charge transfer properties in biologic systems. The core complex and its polymers
reduce nucleic acids as shown by cyclic voltammetry. Preliminary data show the reducing force of the complex can
be regenerated by interaction with hydroxyl fatty acids. The reducing current of the trimers can be directed preferentially
to DNA (not RNA) by the presence of cyanocobalamin. The trimer with cyanocobalamin produces heterochromatin in
Baker's yeast. The yeasts then produce aberrant forms including those undergoing degeneration in a radial sectioning
pattern resembling the cutting of a desert pie. The data suggest the induction of an inward current, starting at
the membrane and converging an DNA.
1.- S.J. Lippard and J.M.Berg, Principles of Bioinorganic Chemistry, University Science Books, Mill Valley
California, (1994 ) , p.141.
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