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"The Effects of a Lipoic Acid/Palladium
Complex on Hippocampal Progenitor Cells"
F.J. Antonawich*. S.M. Fiore and J.N. Davis.
SUNY at Stony Brook,
Stony Brook, NY 11794.
The Lipoic acid/pallacium complex, DNA reductase, appears to be an effective anti-glioblastoma agent in clinical
preliminary studies. The present experiments were aimed to elucidating DNA reductase's effects on CNS cells
still undergoing neurogenesis. We studied the AP31 hippocampal progenitor cell line and 3T3 fibroblasts. The cells
were cultured in DMEM/F12 supplemented with N2 and FGF (20ng/ml). Within 18 hours there was a significant decrease
in the number of progenitor cells on poly-L-ornithine (PORN)/lamine coated plates exposed to DNA reductase (8µM),
however 3T3 cells, on plastic, were unaffected by even the highest tested concentrations. Plating of 3T3 cells
on coverslips further augments the effects on DNA reductase (40µM). Cervical carcinoma cells (HeLa) have
been shown to have this same sensitivity to this glass substrate. Low quality glass, such as coverslip, is highly
charged due to the presence of heavily charged silicates. Poly-L-ornithine promotes cell attachment by positively
charging the surface Therefore, our results suggest that highly charged matrices (e.g. plastic, PORN), normally
seen in growth and in tumor formation, facilitate the effects of DNA reductase, while more neutral surfaces (e.g.
plastic) appear protective. (Supported by Garnett McKeen Laboratories Inc.)
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